ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of prostate stem cell antigen (PSCA) in human pancreatic carcinoma and explore its role in the oncogenesis of pancreatic cancer.</p><p><b>METHODS</b>A pancreatic carcinoma tissue microarray was constructed, which contained 10 normal adult pancreas tissues, 12 chronic pancreatitis tissues and 78 pancreatic carcinomas. Immunohistochemistry was employed to detect the expression of PSCA, and the relation between PSCA expression and the clinicopathological factors of pancreatic carcinoma was analyzed.</p><p><b>RESULTS</b>The positivity rate of PSCA in pancreatic carcinoma was 79.5% (62/78), and PSCA staining was more intense in the malignant cells than in the benign cells (chi2=15.81, P<0.005) and chronic pancreatitis tissues (chi2=11.33, P<0.005). No obvious association was found between PSCA expression and the other variables of pancreatic carcinoma (including gender, age at surgery, tumor grade, and TNM stages).</p><p><b>CONCLUSION</b>The expression of PSCA can be related to the development of pancreatic cancer, but not to the clinicopathological factors of the tumor.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Antigens, Neoplasm , Genetics , Metabolism , Carcinoma, Ductal , Allergy and Immunology , Metabolism , GPI-Linked Proteins , Genetics , Metabolism , Neoplasm Proteins , Genetics , Metabolism , Pancreatic Neoplasms , Allergy and Immunology , Metabolism , Tissue Array Analysis , MethodsABSTRACT
<p><b>OBJECTIVE</b>To investigate the expressions of prostate stem cell antigen (PSCA) and Claudin-4 in human pancreatic carcinoma and to discuss its role in the ontogenesis of pancreatic cancer.</p><p><b>METHODS</b>Pancreatic carcinoma tissue microarray was constructed, containing 100 cores of 10 normal adult pancreas tissues, 12 chronic pancreatitis tissues, and 78 pancreatic carcinomas. The expressions of PSCA and Claudin-4 were detected using immunohistochemical method and the relationship between PSCA and Claudin-4 and the pancreatic carcinoma was analyzed.</p><p><b>RESULTS</b>The positive expression rates of PSCA and Claudin-4 protein in pancreatic carcinoma were 79. % and 88. % respectively. PSCA and Claudin-4 staining were more intense in malignant cells than in chronic pancreatic tissues and normal adult pancreas tissues. No evidence was found for an association between expressions of PSCA and Claudin-4 and other variables, including gender, age at surgery, and tumor grade.</p><p><b>CONCLUSIONS</b>The expressions of PSCA and Claudin-4 are related to the pancreatic carcinomas. PSCA and Claudin-4 play a role in the development of pancreatic cancer, but PSCA and Claudin-4 are not correlated with the clinical pathology of tumor.</p>